Lentiviral vector-EF1a, Puro contains Multiple Cloning Sites (MCS) downstream of the EF1 alpha promoter, and puromycin driven by the PGK promoter as a selection marker. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as 293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be selected by puromycin.
High Protein Expression Levels
Extremely Low recombination rate
Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System
Vector: pLV-EF1a-PGK-Puro
DNA amount 10 µg
Vector Information: Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene: EF1a
Selection Gene: Puromycin
Promoter for Selection Gene PGK
Biosafety Level: BSL-2
Shipped: Room Temperature
Storage: Store at -20°C
Plasmid Map