Lentiviral vector-CMV, RFP contains Multiple Cloning Sites (MCS) downstream of the CMV promoter, and RFP driven by the PGK promoter as a reporter. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as 293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be sorted by RFP fluorescence..
High Protein Expression Levels
Extremely Low recombination rate
Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System
Vector: pLV-CMV-MCS-PGK-RFP
DNA amount 10 µg
Vector Information: Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene: CMV
Selection Gene: /
Promoter for Selection Gene PGK
Biosafety Level: BSL-2
Shipped: Room Temperature
Storage: Store at -20°C
Plasmid Map