Lentiviral vector-CMV, Hyg-2A-GFP

Price
CN¥8,330.00

Lentiviral vector-CMV, Hyg-2A-GFP contains  Multiple Cloning Sites (MCS) downstream of the CMV promoter, and hygromycin/GFP driven by the PGK promoter as a selection marker/reporter. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as 293T to produce VSV-G pseudotyped lentivirus.Transduced stable cells can be selected by hygromycin or sorted with GFP fluorescence.

  • High Protein Expression Levels
  • Extremely Low recombination rate
  • Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System  

  

Vector:pLV-CMV-MCS-PGK-Hyg-T2A-GFP

DNA amount10 µg

Vector Information:Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)

Promoter for Target Gene:CMV

Selection Gene:Hygromycin, GFP

Promoter for Selection GenePGK

Biosafety Level:BSL-2

Shipped:Room Temperature

Storage:Store at -20°C