Lentiviral vector-CMV, Hyg contains Multiple Cloning Sites (MCS) downstream of the CMV promoter, and hygromycin driven by the PGK promoter as a selection marker. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as 293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be selected by puromycin.
- High Protein Expression Levels
- Extremely Low recombination rate
- Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System
Vector:pLV-CMV-PGK-Hyg
DNA amount10 µg
Vector Information:Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene:CMV
Selection Gene:Hygromycin
Promoter for Selection GenePGK
Biosafety Level:BSL-2
Shipped:Room Temperature
Storage:Store at -20°C
Plasmid Map