This strain carries the tetracycline reverse transcriptional activator (rtTA) under the control of the CMV promoter. The rtTA binds to tetracycline operator elements (TetO) in the presence of doxycycline, causing transcriptional activation of genes downstream of TetO.
 

Virus:CMV-rtTA (Puro)

Titer (approx):1 x 108 CFU/ml

Vector Information:Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)

Promoter for Target Gene:CMV

Target Gene / Reporter(s):rtTA

Selection Gene:Puromycin

Biosafety Level:BSL-2

Shipped:Dry ice

Storage:Store at -80°C