Mouse Isotyping Kit – Rapid Mouse Antibody Isotyping XpressCard (ISO-M8a)

Order ISO-M8a: Each kit contains two cassettes, one for IgG1, IgG2a, IgG2b and IgG3, the other κ, λ, IgA, and IgM.

Benefit:

The Rapid Mouse Immunoglobulin Isotyping Kit is a five-minute lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination. It can determine  IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM, as well as κ, λ in one quick test.

Acceptable Sample Types:

include hybridoma culture supernatant and purified monoclonal antibody. Ascites fluid is not recommended since it usually contains multiple immunoglobulins. However, this kit can still be used to determine the relative distribution of the main isotypes in a sample of ascites fluid.

Kit Components:

The isotyping kit has three different sizes with 5, 10 or 20 pouches containing two cassettes per pouch: one cassette is for determining IgG1, IgG2a, IgG2b and IgG3, and the other cassette is for determining κ, λ, IgA and IgM. Both strips contain a control line.

Note:

BALB/c and DBA mice produce IgG2a, not IgG2c. But C57BL/6, C57BL/10, SJL and NOD produce IgG2c, not 2a*.

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