The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are frequently silenced in advanced cancer and have been implicated in epithelial to mesenchymal transition (EMT) and tumor invasion by targeting the transcriptional repressors of E-cadherin, ZEB1 and ZEB2. This lentivirus expresses miR-200a when Tetracycline reverse transcriptional activator (rtTA) binds to Tetracycline operator elements (TetO) in the presence of doxycycline. 
 

Virus:Inducible tet-on miR-200a

Titer (approx):1 x 108 TU/ml

Vector Information:Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)

Promoter for Target Gene:TRE

Target Gene / Reporter(s):miR-200a

Species:
Mouse 

Selection Gene:Puromycin

Biosafety Level:BSL-2

Shipped:Dry ice

Storage:Store at -80°C